To examine whether serial transmission of brain material containing PrPres leads to clinical prion disease, brain material from animals inoculated with dgPMCAb-derived PrPres induced either by α-synuclein WT fibrils, the lysates of HeLa cells expressing α-synuclein A30P, or non-seeded dgPMCAb-derived material were used for the second passage (Table 1). This evidence concerns the gene SNCA and prion disease.