To evaluate whether the moderate basal NF-κB activity of U251-NF-κB-GFP-Luc cells is helpful in detection of anti-inflammatory molecules or drugs, we first tested a range of concentrations of Bay11-7082, a general IKK inhibitor (poorly selective for IKK1 or IKK2) proposed as a potential compound for glioma treatment [35] without an additional proinflammatory trigger. This evidence concerns the gene CHUK and central nervous system cancer.