In this study, para-nitro enhanced the anti-colon cancer activity of CAPE, and the present data showed that CAPE-pNO2 is more effective than CAPE in inducing colon cancer cell death, apoptosis and cell cycle arrest in the G0/G1 phase by regulating the relative proteins in the P53 pathway and inhibiting tumour growth. The gene discussed is TP53; the disease is colonic neoplasm.