Western blot analyses were performed to compare HIF-1α protein expression in four representative PCa cell lines (androgen-receptor-negative PC-3 and DU145, androgen-responsive LNCaP, and castration-resistant 22RV1) and in two non-malignant prostatic epithelial cell lines (RWPE-1 and BPH1). Here, AR is linked to posterior cortical atrophy.