To investigate the effect of TLR3 agonist on chicken lymphoma, the Marek’s disease lymphoma-derived chicken cell line MDCC-MSB1 cells and the avian leukosis virus (ALV) lymphoma-derived chicken cell line DT40 cells were cultured with 1 μg/ml, 10 μg/ml or 100 μg/ml dsRNA analog poly (I:C) for 24 h. The gene discussed is TLR3; the disease is lymphoma.