The classification of the TMA staining was based on the percent of positive cells and staining intensity in our study, while c-Jun and Fra-1 also present in cytoplasm, for example, in certain types of cancers including breast, lung and thyroid cancer cytoplasmic Fra-1 over-expression has been reported, and there is also evidence showing that Fra-1 and c-Fos support growth of human malignant breast tumors by activating membrane biogenesis at the cytoplasm50. The gene discussed is JUN; the disease is thyroid cancer.