UPK2 and urinary bladder cancer: In order to extend Ad5 application for CAR negative expression bladder cancer we construct bladder cancer-specific chimeric type viruses Ad5/F11p-PSCAE-UPII-E1A according to replace the fiber gene of pAdEasy-1 with Ad5/F11p concluding Ad5 fiber tail domain and Ad11p fiber shaft and knob domains.