EGFR and infection: When we looked at the distribution of Rab11-Fip2 during the early phase of infection, we detected 95% colocalization of Rab11-Fip2 with EGFR/PI3P-positive endosomes containing chlamydial EBs at 5 min p.i., and this was confirmed by live-cell imaging of cells expressing GFP-Fip2 and either EGFR-mCherry or mCherry-2xFYVE (Fig 4A and 4B, S4, S5 and S6 Movies).