NFKB1 and infection: To assess whether early inhibition of NF-κB-p65 nuclear translocation by ORFV073 involves de novo viral protein synthesis in the infected cells, OFTu cells were pre-treated with the protein synthesis inhibitor cycloheximide (CHX) for 30 min followed by infection with OV-IA82, OV-IA82Δ073 or OV-IA82RV073Flag for 1 h in presence of the drug.