KRAS and neoplasm: The quality, depth-of-coverage and accuracy metrics of the new HaloPlex-WES assay were first established using HapMap DNA specimens (NA12878/NA19240) and 57 matched normal/tumour samples positive for any of mutation in five genes (KRAS, BRAF, JAK2, EGFR and HER2/neu), according to NYS-DOH NGS guidelines for somatic genetic variant detection (Figure 1c).