After statistical analysis confirmed that: The gene expression of TLRS (TLRS = TLR1 + TLR2 + TLR3 + TLR4 + TLR5 + TLR6 + TLR7 + TLR8 + TLR9) in severe and moderate dysmenorrhea group was significantly higher than that in minimal dysmenorrhea group in EU and EC (P < 0.05 or P < 0.01). The gene discussed is TLR9; the disease is Dysmenorrhea.