We provided further evidence that Kapβ2-mediated ciliary localization of Gli is essential for Hh pathway activity in multiple physiologically relevant contexts and depletion of Kapβ2 affected the growth of SmoM2-driven medulloblastoma cells cultured in vitro, suggesting that blockage of Kapβ2-mediated Gli ciliary localization may serve as a new strategy to treat Hh-driven cancers such as basal cell carcinoma (BCC) and medulloblastoma. Here, GLI1 is linked to cancer.