IFNA1 and infection: Supernatants from trophoblasts and HC infected with ZIKV PRVABC59 were unable to induce CPE in Vero cells (which are IFN deficient), and 1 μM of ruxolitinib, a supra-physiological concentration of drug added to HC or trophoblast cultures during infections, resulted in production of virions that were able to confer virally induced CPE in uninfected Vero cells using HC, but not trophoblast supernatants (Figure 3).