To further validate the role of COL1A1 in the radioresistance of cervical cancer, COL1A1-shRNA and activation plasmids were used to constructed stably transfected in Hela and CaSki cells, and these cells were irradiated with 4 Gy, then these cells were tested for colony formation (Fig. 4b). The gene discussed is COL1A1; the disease is cervical carcinoma.