Next, expression levels of eight EWS-FLI1 associated target genes (GLI1, NEX2.2, CyclinD, c-MYC, NR01B, IGF1R, EZH2, and CD99) which were known to be upregulated, and three genes (FOXO1, IGFBP3, and LOX) known to be down regulated in Ewing’s sarcoma was assessed. This evidence concerns the gene MYC and Ewing sarcoma.