GLIPR1 and lung adenocarcinoma: To examine whether the epigenetic status of GLIPR1 in DDP-sensitive and -resistant human lung adenocarcinoma A549/DDP cells was responsible for the high expression of GLIPR1 in A549/DDP cells [9, 15, 20], bisulfite sequencing primers were designed to amplify a 264 bp region 5′ of the transcription start site containing five CpG sites as previously reported.[15] GLIPR1 bisulfite sequencing data were obtained on a minimum of 5 clones prepared from each of both A549 and A549/DDP cells (Fig 8A).