During development of symptomatic HD, the appearance of mutant HTT aggregates precedes the death of medium spiny neurons located in the striatum.22 Therefore, we addressed the potential of our AAV5-miHTT-451 and AAV5-miSNP50T-451 vectors to suppress DARPP-32-associated neuronal dysfunction by performing IHC against DARPP-32 (Figures 4c and d). Here, PPP1R1B is linked to Huntington disease.