In order to test whether the partial and systemic autophagy impairment of Atg4b-null mice might also predispose to experimentally induced type-I diabetes, we next decided to treat WT and autophagy-deficient mice with streptozotocin (STZ), Thus, mice were administered a single dose of STZ and after 3 days, blood glucose levels and glucose tolerance were evaluated. Here, ATG4B is linked to type 1 diabetes mellitus.