ATG4B and Insulin resistance: In WT mice, this regimen fails to induce a major weight gain, yet causes hepatic steatosis and insulin resistance.23, 24 Unexpectedly, but in accord with our previous results, atg4b−/− mice exhibited a steady weight gain that reached ~50% after 100 days of sucrose treatment, which was significantly higher that what was observed in WT controls under this same regimen (Figure 1d).