In WT mice, this regimen fails to induce a major weight gain, yet causes hepatic steatosis and insulin resistance.23, 24 Unexpectedly, but in accord with our previous results, atg4b−/− mice exhibited a steady weight gain that reached ~50% after 100 days of sucrose treatment, which was significantly higher that what was observed in WT controls under this same regimen (Figure 1d). Here, ATG4B is linked to fatty liver disease.