Correspondingly, immunostaining analysis of PI3K110a, Akt Thr308, Akt Ser473, Akt and NF-kB were performed in harvested tumour tissues, displaying similar results as western blotting in that PI3K110a, Akt Thr308, Akt Ser473 and NF-kB were decreased by LY294002 treatment or Akt siRNA (Figure 6g). The gene discussed is AKT1; the disease is neoplasm.