To test the effects of in vitro antioxidant supplementation, Petro et al. divided male rats with experimentally induced thromboembolic stroke into four groups: normal rats, untreated rats with stroke, rats receiving tissue plasminogen activator (tPA) only, and rats receiving tPA + CAT/SOD (loaded on nanoparticles) at three hours post stroke. Here, SOD1 is linked to stroke disorder.