Furthermore, genetic studies of Gata4 in mice have definitively demonstrated that Gata4 is required for normal lung and diaphragm development, and that Gata4 loss-of-function mutations cause CDH (Ackerman et al., 2007; Jay et al., 2007; Merrell et al., 2015). Here, GATA4 is linked to congenital diaphragmatic hernia.