Janeway et al. used a combination of SDHX sequencing and SDHB IHC and/or immunoblotting to determine the SDH status of a series of GIST, including 34 sporadic RTK-wild type tumors (pediatric and young adult), 18 KIT-mutated tumors, and 5 NF1-associated tumors [54]. The gene discussed is SDHB; the disease is gastrointestinal stromal tumor.