In the present study, a culture of mammary epithelial MCF10A cells with CM from breast cancer cells MDA-MB-231 and MDA-MB-453 (termed 231-CM and 453-CM) resulted in a morphological change from epithelial-shaped to spindle-shaped, similarly to TGF-β-induced EMT (Figure 1a). This evidence concerns the gene TGFB1 and breast carcinoma.