Couthouis et al. (2012) also scanned the EWSR1 gene for mutations in a different cohort of ALS patients, this time focusing only on the exons that encoded the C-terminal RGG- and PY-motif regions, as the equivalent regions in FUS and TDP43 are hotspots for ALS-associated mutations. This evidence concerns the gene EWSR1 and amyotrophic lateral sclerosis.