BDNF and insomnia measurement: The effect of tranquilizing and allaying excitement needling method treatment on the expression of BDNF and SYP in the sleep deprivation was a critical evidence that tranquilizing and allaying excitement needling method improved learning and memory ability impaired by insomnia and it gave us some evidence in the mechanism of clinical treatment of insomnia by tranquilizing and allaying excitement needling method.