Due to the observation that some human cancer cell lines, including AsPC-1 (human pancreas adenocarcinoma ascites metastases), DLD-1 (human colorectal adenocarcinoma) and Capan-2 (human pancreatic ductal adenocarcinoma), exhibit high level of endogenous AP activity which cannot be inactivated by at 65 °C usually used for removing background AP activity from host cells (data not shown), we examined the binding of AP-TRAIL to cultured human pancreas adenocarcinoma cell lines after heat treatment of the cells at 100 °C for 10 min. Here, TNFSF10 is linked to colorectal adenocarcinoma.