Based on an observed change in tumor cell morphology we next initiated quantitative analysis comparing the morphology of untreated, miR modulated and NSCLC cells stimulated with TGF-β, which is a strong inducer of EMT.7 We determined that miR-151a is an inducer of a mesenchymal-like morphology of A549 cells (spindle-formed, far separated cells), relative to untreated and miR control A549 cells, though not to the extent of TGF-β (Figure 4a, miR-151a: mesenchymal=77.7%, epithelial=17.7%, undefined=4.6% and Supplementary Figure S2). Here, TGFB1 is linked to neoplasm.