In this study, a well-established HCV produced in theJ6/JFH1-Huh7.5.1 culture system was used to infect cultured PTHs and tree shrews.The in vitro results showed that HCV genomic RNA and anHCV-specific protein (NS5A) could be detected in the PTH cell culture from3–15 days post-infection, in agreement with the report that indicatedthat PTHs are susceptible to HCV infection [7]. The gene discussed is TCF4; the disease is infection.