To first characterize efficacy and selectivity of HisDianthin‐EGF on pancreatic cell lines and the enhancer effect of SO1861, cytotoxicity of HisDianthin‐EGF in the absence and presence of SO1861 was determined on the EGFR‐overexpressing pancreatic carcinoma cell lines BxPC‐3 and MIA PaCa‐2 as well as on off‐target NIH‐3T3 cells. This evidence concerns the gene EGFR and exocrine pancreatic carcinoma.