USP6 and neoplasm: To further confirm the results under tumour-like conditions, we employed a transgenic zebrafish model Tg(fabp10:rtTA2s-M2; TRE2:EGFP-krasG12V) (Yan et al., 2015), in which krasG12V expression and the hepatic hyperplasia phenotype can be chemically induced by adding doxycycline to the zebrafish liver from 2 dpf to 6 dpf (Fig. 4A).