To further confirm the level of CCDC109B in normal brain tissue samples and different grades glioma tissues, we searched publicly available databases, Rembrandt, TCGA, Chinese Glioma Genome Atlas (CGGA) and found a relatively higher mRNA level of CCDC109B in HGG in contrast to low grade gliomas (LGG; WHOI-II) and normal brain tissues (P < 0.001, Fig. 1c). Here, MCUB is linked to glioma.