To evaluate the role of RhoJ in melanoma development, constitutive RhoJ knockout (KO) mice were crossed with a previously described autochthonous mouse model of melanoma that carries a Tyr:CreERT2 allele, a BrafCA allele, and one or two copies of a Ptenlox4-5 allele [18] (S1A Fig). The gene discussed is RHOJ; the disease is melanoma.