The distal region of the MLH1 promoter, which corresponds to the 5′ shore of the CpG island and is adjacent to the identified MLH1 cDMC (chr3:37034084), was shown methylated in a colon cancer-specific manner, whereas the proximal region residing within the CpG island was equally unmethylated in all cell lines examined. This evidence concerns the gene MLH1 and malignant colon neoplasm.