Although the MLH1 cg11291081 hypermethylation turned out to be tissue-specific, instead of cancer-specific, it did not mean that our TBPseq failed its task, because the method succeeded in pointing the cg11291081 anyway as a differential marker specific to the colon cancer cell lines against the other cancer cell lines. The gene discussed is MLH1; the disease is malignant colon neoplasm.