PPARA and Insulin resistance: Furthermore, moderate (~20%) DNA methylation changes were induced by the paternal low-protein diet, including changes in methylation of the key regulator of lipid metabolism, Ppara. In another mouse study, the induction of a prediabetic state in male ancestors by combination of streptozotocin and high-fat diet caused glucose intolerance, insulin resistance and enhanced diabetes risk in their F1 and F2 offspring [56].