We found that five factor srRNA (5F-srRNA), including OCT4, KLF4, SOX2, GLIS1 and c-MYC (OKSi-GM), significantly increased iPSC generation in six different adult human fibroblasts, including old adult fibroblasts and a cardiomyopathy patient donor. The gene discussed is GLIS1; the disease is cardiomyopathy.