To explore miR-181d targets that might explain its oncogenic role in CRC, we identified CRY2 and FBXL3 as two direct targets of miR-181d based on the following evidence that (i) miR-181d overexpression significantly decreased CRY2 and FBXL3 protein expression; (ii) CRY2 and FBXL3 and miR-181d expression levels were negatively correlated; and (iii) 3′-UTR-luciferase reporter activities of both CRY2 and FBXL3 were suppressed by miR-181d overexpression, although this effect was not observed after mutating the miR-181d seed-binding sequence. This evidence concerns the gene FBXL3 and colorectal carcinoma.