PGK1 and hepatocellular carcinoma: According to the results of endogenous expression of PGK1 in HCC cell lines, SNU182 and JHH5 were selected for the overexpression of PGK1, SNU182/Vector and JHH5/Vector were used as the normal control; SNU449 and HCCLM3 were selected for knocking down PGK1, then we examined the transfection efficiency by Western blotting with SNU449/NC and HCCLM3/NC as normal controls (Figure 1B).