However, administration of lenti-shLFAR1 greatly reduced CCl4-induced liver fibrosis, as demonstrated by macroscopic examination, H&E staining, Sirius red staining, TUNEL staining, IHC and western blot for α-SMA and collagen1 (Fig. 4f, g and Supplementary Fig. 9e). The gene discussed is ACTA1; the disease is Hepatic fibrosis.