As shown in Fig. 1a and Supplementary Fig. 1a, macroscopic examination, hematoxylin-eosin (H&E) staining, Sirius red staining, immunofluorescent assay for collagen1 and immunohistochemistry (IHC) for α-SMA and collagen1 confirmed the occurrence of liver fibrosis. The gene discussed is ACTA1; the disease is Hepatic fibrosis.