In Boyden chamber invasion assays, a significant increase in cell invasion was noted in cells depleted of AIM1 by multiple sh-AIM1 constructs in prostate epithelial cells (RWPE-1, 957) as well as in prostate cancer cell lines (VCaP and PC3) (Fig. 5d and Supplementary Fig. 3). This evidence concerns the gene CRYBG1 and Familial prostate cancer.