Conversely, we also co-transfected gastric cancer cells with the following synthetic RNAs/plasmids: (1) pre-miR-control and control plasmid, (2) pre-miR-control and MEG2 overexpression plasmid with full-length ORF lacking the miR-181a-5p-responsive 3′-UTR, (3) pre-miR-181a-5p and control plasmid, and (4) pre-miR-181a-5p and MEG2 overexpression plasmid. The gene discussed is PTPN9; the disease is gastric cancer.