To establish the role of miR-183-5p in cardiac hypertrophy and heart failure and to explore the relationship between miR-183-5p and AGGF1 during the process, we performed TAC procedures for both WT and Aggf1+/− mice, which were followed by intramuscular injection of Ago-miR-183-5p and control Ago-miR-NC. Here, FBXW7 is linked to heart failure.