The only differences between WT and ALD1 KO infections are again observed 6 d.p.i. While the percentage of infected cells in WT at 6 d.p.i. was quite low (5%), there was almost a complete absence of infected cells in ALD1 KO, with only one cell containing a single amastigote observed (Fig 5B, right panel). This evidence concerns the gene ABCD2 and infection.