TPCN1 and infection: Consistent with this observation, Tpc1:GFP was also highly expressed in Δatg1 and Δatg8 mutants impaired in autophagy, suggesting that the expression of TPC1 is de-repressed as a consequence of the inability to carry out autophagy and may therefore be an upstream positive regulator of infection-associated autophagy during appressorium maturation (Fig 9).