Alternatives may be to analyze samples via an immunofluorescence staining approach [16], which may provide higher sensitivity and a broader dynamic range relative to immunohistochemistry methods, or by fluorescence in situ hybridization (FISH), which can detect EGFR gene amplification and copy number gain, a measure potentially more closely associated with poor prognosis in ovarian cancer [13, 20, 35]. Here, EGFR is linked to ovarian carcinoma.