Regulatory T cells were characterized by the CD4+CD25+FOXP3+ phenotype as shown in the Figure 6A. An increased number of Treg cells was observed in the lungs of Nlrp3−/−, Casp1/11−/−, and Asc−/− at 4 and 10 weeks of infection, compared with WT mice, which also showed increased number of these cells, although lower than NLRP3 components deficient mice (Figure 6B). The gene discussed is CD4; the disease is infection.