TWIST1 and neoplasm: These cell lines, 4T1t and 4T1m, had stronger tumor initiating and invasive capacities compared to 4T1 cells, and elicited an EMT associated with upregulation of EMT-promoting transcription factors; i.e., Twist1/2 and Zeb122, as well as factors related to stemness, such as Hes1, Sox2 and Oct3/425–27.