To functionally test the possibility that tumor-intrinsic PPARγ/RXRαS427F/Y pathway activity may influence localization of CD8+ T cells, we subcutaneously implanted murine bladder tumor MBT2 cells engineered to overexpress RXRαWT or RXRαS427F (Supplementary Fig. 23) into syngeneic mice and assessed basal tumor infiltration of CD8+ T cells once the tumors reached ~300 mm3. The gene discussed is CD8A; the disease is neoplasm.