To further investigate the potential role of fibulin-3 in osteosarcoma cell proliferation and invasion, we decreased the expression of fibulin-3 in the highly invasive subclone HOS-1, and increased fibulin-3 expression in the low invasive subclone HOS-29 by lentivirus transfection. The gene discussed is EFEMP1; the disease is osteosarcoma.