The notes linked to cpa and cpe laboratory results highlight that the presence of the DNA of the C. perfringens alpha toxin over 300,000 copies/g could be implicated in the clinical signs but, in case of cpe copies under the limit or absent, as in our case, it is unlikely that cpa is the cause of diarrhoea and definitely not cpe. All these aspects led us to speculate that a co-infection was responsible. This evidence concerns the gene CPA1 and Diarrhea.