Argininosuccinate synthetase(control/T1DM = 2.74, T1DM/AFB1 = 1.08), argininosuccinate synthase (control/T1DM = 1.73, T1DM/AFB1 = 0.86)) and arginase-1(control/T1DM = 1.68, T1DM/AFB1 = 0.96), participants in the urea cycle, were decreased in the T1DM group in our study. The gene discussed is ASS1; the disease is type 1 diabetes mellitus.